Shannon McCook
48-Hour Chick Embryo: Brightfield, 2006

Photomicrograph; magnification approximately x12 at capture; direct digital capture using a SPOT II camera

Biomedical Photographic Communications, Rochester Institute of Technology, Rochester, New York, United States

This photograph is a chicken embryo injected with India ink at the 48th hour of development. The whole organism was mounted on a slide for examination, which would be described as a whole mount. This photograph is comprised of over 250 individual images used to create a map of the specimen. Because of thickness and the requirement for image depth of field, approximately 23 images were exposed at varying image depths across each 1-micrometer distance of the embryo to provide for maximum depth of field and resolution in the final photograph. These images were then digitally merged using Adobe PhotoShop to create the final composite picture.

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