Medical Science Camp Week 2
Antibiotic Discovery and Enzymes
July 18 to July 22
Camp Description
Have you ever heard your doctor say: I am sorry Sir/Madam, but the antibiotic that was prescribed is not working due to antibiotic resistance; we have to identify a new antibiotic that will work for you?" The discovery of new compounds that inhibit bacterial growth is an everlasting quest. In order to discover new antibiotics to fight bacterial infections, researchers must first discover enzymes that are present in bacteria that are not associated with humans or animals. Once the bacterial enzyme is identified, researchers can begin the process of discovery by identifying compounds that are able to block enzyme activity which will prevent bacterial infection.
Over the course of one week, students will perform hands on experiments relating to the detection, isolation and the measurement of enzyme activity from bacteria.
Sample Investigations
Students will perform experiments to observe the action of the enzyme in a living organism to answer the following question? Is enzyme X a good target for antibiotic development? Students will also utilize various computer programs that researchers use in this endeavor.
- BLAST AWAY: Students will be introduced to Basic Local Alignment Search Tool (BLAST) computer program that is used compare proteins from different organisms.
- Enzyme Purification and Detection: Students will perform enzyme purification and detect the purified enzyme by protein electrophoresis.
- Functional complementation: Students will perform this critical test to see if the enzyme in question is absolutely essential for bacteria growth.
Other Notes
Students are expected to bring lunch.
Snacks will be provided during the day.
