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Center For Applied and Computational Mathematics

Liquid Structure of Concentrated Eye Lens Protein Mixtures

Liquid Structure
Faculty: George Thurston
  David Ross
  John Hamilton

Summary:

In this interdisciplinary work we are studying multicomponent, aqueous protein mixtures that are important for understanding the cytoplasm of the lens of the eye. A number of mathematical projects are essential for this work including (i) statistical-thermodynamic modeling of the free energy and liquid structure of globular protein mixtures, (ii) the interpretation of light, neutron and X-ray scattering data relevant to liquid structure and thermodynamics, (iii) statistical thermodynamic Monte Carlo and molecular dynamics simulation of concentrated protein mixtures, and (iv) study and modeling of the potentials of mean force between protein molecules.

Poster:

Publications:

  1. Cataract-associated mutant E107A of human gammaD-crystallin shows increased attraction to alpha-crystallin and enhanced light scattering, Banerjee, P. R., Pande, A., Patrosz, J., Thurston, G. and Pande, J., Proc. Natl. Acad. Sci. U.S.A., 50, 3279-3281.(Cover article, Epub. December 20, 2010),

  2. Liquid-liquid Phase Separation and Static Light Scattering of Concentrated Ternary Mixtures of Bovine α and γB Crystallins, G. M. Thurston, Journal of Chemical Physics, 124, 134909 (2006).

  3. New insight into cataract formation: enhanced stability through mutual attraction", A. Stradner, G. Foffi, N. Dorsaz, G. M. Thurston, and P. Schurtenberger, Physical Review Letters, 99 (19): Art. No. 198103 (2007).

  4. Colloidal Characterization and Thermodynamic Stability of Binary Eye Lens Protein Mixtures, N. Dorsaz, G. M. Thurston, A. Stradner, P. Schurtenberger and G. Foffi, J. Phys. Chem. B, 113 (6):1693-1709 (2009).