Welcome to the Confocal Microscopy Lab at RIT. This lab provides faculty and students with a multidisciplinary imaging research and training facility centered around confocal microscopy.
The laboratory's core mission is to enhance research capabilities, provide an environment that promotes multidisciplinary collaborations, and involve undergraduate and graduate students to support their training and preparation.
The Confocal Microscopy Lab is located in Gosnell A334.
Provide a multidisciplinary confocal microscopy research and training environment to enhance student/faculty interactions and providing a resource to the general community.
Provide workshops to train new faculty and student users.
Supporting confocal imaging in current courses to expose RIT students to state-of-the-art instrumentation.
Providing a multidisciplinary research-based course to foster new research ideas and collaborations and to facilitate the integration of research and education.
Science Lab, the knowledge portal of Leica Microsystems, offers scientific research and teaching material on the subjects of microscopy. The content is designed to support beginners, experienced practitioners and scientists alike in their everyday work and experiments. Explore interactive tutorials and application notes, discover the basics of microscopy as well as high-end technologies – become part of the Science Lab community and share your expertise!
Simultaneous detection of up to 5 fluorophores from 400nm to 800nm using prism mirror design for superior transparency (95%) and efficiency
Set emission bandwidth as small (specific) as 5nm and as large (efficient) as 300nm e.g. ECFP emission peak at 475nm480nm, broad-spectrum DAPI at 400nm-700nm
Set detection window wavelength minimum and maximum to the nanometer eg. FITC alone at 495nm-530nm or simultaneous FITC and TRITC at 495nm-514nm and 563nm611nm respectively.
Spectral imaging (ie. separation) of spectrally similar yet intensity dissimilar fluorophores through individual adjustment of amplification gain (voltage) control on MULTIPLE detectors eg. CFP-YFP, GFP-YFP
Separation of fluorophores without reference spectra in most cases (simultaneous - optical, sequential-optical, or channel-mathematical)
Compatible with fast-RS and TS-scanners for FAST spectral imaging!
Once you have read the information and are ready to begin imaging your project, please fill out provided application and e-mail to Hyla Sweet. Once the application has been processed an imaging session can be scheduled.
The RIT confocal microscopy lab is supported through the National Science Foundation Major Research Instrumentation Program (#1126629), the RIT Office of the Vice President for Research, the Kate Gleason College of Engineering, and the RIT College of Science. All images on this site were produced in the CML.