Michael Gleghorn Headshot

Michael Gleghorn

Assistant Professor

School of Chemistry and Materials Science
College of Science
mlgsch@rit.edu
585-475-5528
Office Location
GOS-2290

Michael Gleghorn

Assistant Professor

School of Chemistry and Materials Science
College of Science

Education

BS, Clarion University; Ph.D., Pennsylvania State University

mlgsch@rit.edu
585-475-5528
Personal Links
Personal Website

Select Scholarship

Michel, L.V., Kaur, R., Gleghorn, M.L., Holmquist, M., Pryharski, K., Perdue, J., Jones, S.P., Jackson, N., Pilo, I., Kasper, A., Labbe, N., Pichichero, M. (2022) Haemophilus influenzae Protein D antibody suppression in a multi-component vaccine formulation. FEBS Open Bio. Oct 20;. doi:10.1111/feb4.13498. PMID: 36263849. PubMed link. Journal link.

Jones, S.P.*, Cook, K.H.*, Holmquist, M.L., Almekinder, L.J., Delaney, A.M., Charles, Ryhl, Labbe, N., Perdue, J, Jackson, N., Pichichero, M.D., Kaur, R., Michel, L.V., Gleghorn, M.L. (2022) Vaccine target and carrier molecule nontypeable Haemophilus influenzae protein D dimerizes like the close Escherichia coli GlpQ homolog but unlike other known homolog dimers. PROTEINS: Structure, Function, and Bioinformatics. PMID 36065600 PubMed link. Journal link.

*equal contribution

Jones S.P., Goossen C., Lewis, S.D., Delaney, A.M., Gleghorn, M.L. (2022) Not making the cut: Techniques to prevent RNA cleavage in structural studies of RNase-RNA complexes. JSBX. Mar 11;6:100066. doi: 10.1016/j.yjsbx.2022.100066. PMID: 35340590 PubMed link. Journal link.

Pickard M.A.G, Brylow K.B., Cisco L.A., Anecelle M.R., Pershun M.L., Chandrasekaran A.R., Halvorsen K., Gleghorn M.L. (2020) Parallel poly(A) homo- and hetero-duplex formation detection with an adapted DNA nanoswitch technique. RNA. May 15;. doi: 10.1261/rna.075408.120. [Epub ahead of print] PMID: 32414856. PubMed link. Journal link.

Visentin S., Cannone G., Doutch J., Harris G., Gleghorn M.L., Clifton L., Smith B.O., Spagnolo L. (2020) A multipronged approach to understanding the form and function of hStaufen protein. RNA. Mar;26(3):265-277. doi: 10.1261/rna.072595.119. Epub 2019 Dec 18. PubMed PMID: 31852734; PubMed Central PMCID: PMC7025507. PubMed link. Journal link.

Cho H.*, Rambout, X.*, Gleghorn, M.L.*, Nguyen, P.Q.T., Phipps, C.R., Miyoshi, K., Myers, J.R., Kataoka, N., Fasan, R., Maquat, L.E. (2018) Transcriptional coactivator PGC-1α contains a novel CBP80-binding motif that orchestrates efficient target gene expression. Genes & Development. 32(7-8): 555-567. PMCID: PMC5959238 *equal contribution PubMed link. Journal link.

“Compositions and Methods for Inhibiting CBP80 Binding to PGCI Family of Co-activators”. U.S. Patent Application No. 16/060,109, filed on June 7th, 2018

Gleghorn M.L., Zhao J., Turner D.H., Maquat L.E. (2016) Crystal structure of a poly(rA) staggered zipper at acidic pH: evidence that adenine N1 protonation mediates parallel double helix formation. Nucl. Acids. Res. 44 (17): 8417-8424. PMCID: PMC5041459 PubMed link. Journal link.

Gleghorn M.L. and Maquat L.E. (2014) 'Black sheep' that don't leave the double-stranded RNA-binding domain fold. Trends Biochem. Sci. 39 (7): 328-40. PMCID: PMC4077987 PubMed link. Journal link.

Gleghorn M.L., Gong C., Kielkopf C.L., Maquat L.E. (2013) Staufen1 dimerizes through a conserved motif and a degenerate dsRNA-binding domain to promote mRNA decay. Nat. Struct. Mol. Biol. 20 (4): 515-24. PMCID: PMC4096160 PubMed link. Journal link.

Park, E., Gleghorn, M.L., Maquat, L.E. (2013) Staufen2 functions in Staufen1-mediated mRNA decay by binding to itself and its paralog and promoting UPF1 helicase but not ATPase activity. Proc Natl Acad Sci U.S.A. 110 (2): 405-12. PMCID: PMC3545820 PubMed link. Journal link.

Gleghorn, M.L., and Maquat, L.E. (2011) UPF1 Learns to Relax and Unwind. Mol Cell 41: 621-623. PMID: 21419337 PubMed link. Journal link.

Chen, Y., Basu, R., Gleghorn, M., Murakami, K., Carey, P. (2011) Time resolved events on the reaction pathway of transcript initiation by a single subunit RNA polymerase: Raman crystallographic evidence. J. Am. Chem. Soc. 133 (32): 12544-12555. PMCID: PMC3154994 PubMed link. Journal link.

Gleghorn, M.L., Davydova, E.K., Basu, R., Rothman-Denes, L.B., Murakami, K.S. (2011) X- ray crystal structures elucidate the nucleotidyl transfer reaction of transcript initiation using two nucleotides. Proc Natl Acad Sci U.S.A. 108: 3566-3571. PMCID: PMC3048110 PubMed link. Journal link.

Gleghorn, M.L., Davydova, E.K., Rothman-Denes, L.B., Murakami, K.S. (2008) Structural basis for DNA-hairpin promoter recognition by the bacteriophage N4 virion RNA polymerase. Mol. Cell 32:707-717. PMCID: PMC2639713 PubMed link. Journal link.

Currently Teaching

CHEM-493
Chemistry Research
1 - 3 Credits
This course is a faculty-directed student project or research in chemistry that could be considered of an original nature.
CHEM-495
Advanced Chemistry Research
1 - 3 Credits
This course is a faculty-directed student project or research involving laboratory work, computer modeling, or theoretical calculations that could be considered of an original nature. The level of study is appropriate for students in their final two years of study.
CHEM-780
Chemistry Project
1 - 4 Credits
Chemistry project accomplished by the MS student for an appropriate topic as arranged between the candidate and the project advisor.
CHEM-790
Research & Thesis
1 - 6 Credits
Dissertation research by the candidate for an appropriate topic as arranged between the candidate and the research advisor.
CHMB-402
Biochemistry I
3 Credits
This course introduces the structure and function of biological macromolecules and their metabolic pathways. The relationship between the three-dimensional structure of proteins and their function in enzymatic catalysis will be examined. Membrane structure and the physical laws that apply to metabolic processes will also be discussed.
CHMB-405
Biochemistry Lab
3 Credits
An introduction to the theory and practice of modern experimental biochemical laboratory techniques and concepts. The weekly two-hour lecture provides a theoretical framework for the course and includes a discussion of the properties of biomolecules and how those properties are exploited in the separation and characterization of the molecules. Practical laboratory techniques include the preparation of buffers, centrifugation, chromatography, electrophoretic methods, and UV-visible spectrophotometry as applied to the isolation and characterization of proteins and nucleic acids. The manipulation of genetic material in E. coli will also be executed. This course will be offered in a writing intensive format where the students will write and submit the different sections found in scientific papers (abstract, introduction, materials and methods, results, discussion, conclusions, references, figures, tables) in an iterative fashion that will include regular feedback from the instructor.
CHMB-493
Biochemistry Research
1 - 3 Credits
This course is a faculty-directed student project or research in biochemistry that could be considered of an original nature.
CHMB-495
Advanced Biochemistry Research
1 - 3 Credits
This course is a faculty-directed student project or research involving laboratory work, computer modeling, or theoretical calculations that could be considered of an original nature. The level of study is appropriate for students in their final two years of study.
CHMB-704
Advanced Nucleic Acids Biochemistry; Structure and Function
3 Credits
This course will cover nucleic acid structures as determined by NMR and X-ray crystallography and nucleic acid catalysis, especially that of ribozymes. Genomics, specifically whole-genome sequencing papers, will be analyzed. Current RNA topics including the RNA World, Ribozymes, RNAi, and Riboswitches will be discussed. Current DNA topics including Lateral/Horizontal DNA Transfer, Genome Duplication, Alternate Gene Expression and Synthetic Life will also be discussed.
CHMG-141
General & Analytical Chemistry I
3 Credits
This is a general chemistry course for students in the life and physical sciences. College chemistry is presented as a science based on empirical evidence that is placed into the context of conceptual, visual, and mathematical models. Students will learn the concepts, symbolism, and fundamental tools of chemistry necessary to carry on a discourse in the language of chemistry. Emphasis will be placed on the relationship between atomic structure, chemical bonds, and the transformation of these bonds through chemical reactions. The fundamentals of organic chemistry are introduced throughout the course to emphasize the connection between chemistry and the other sciences.

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