Chemistry Seminar: Effect of in-vivo Protein-Protein Interactions on Antibody Production in Mice
The Effect of in-vivo Protein-Protein Interactions on Antibody Production in Mice
Jonathan Dominguez
Chemistry MS Candidate
School of Chemistry and Materials Science, RIT
Abstract:
Nontypeable Haemophilus influenzae (NTHi) is the predominant pathogens that inhabit and colonize the human nasopharynx. NTHi is also leading cause of acute otitis media (AOM), a bacterial infection in the middle ear that disproportionately affects young children. AOM is one of the most common ailments in children and for which antibiotics are prescribed, thus underling the need for a vaccine against NTHi. Due to advances in molecular and genetic technologies, protein-based vaccines are becoming easier to manufacture. However, there are significant obstacles to creating an effective protein vaccine, which include identifying protective protein vaccine candidates and formulating a vaccine with no adverse side effects. Here, we describe a case study in which two leading protein vaccine candidates from NTHi (OMP26 and Protein D) are combined in a single vaccine formulation, leading to antibody suppression of one of the candidates. We will present work done to evaluate the inter-protein interactions between OMP26 and Protein D, as well as a description of strategies to overcome the antibody suppression.
Speaker Bio:
Jonathan Dominguez is from Chicago, Illinois. He came to RIT in 2017 and received a Bachelor’s degree in Biomedical Sciences last semester 2020. During the summer of 2019, he had REU at RIT and began working with Todd Pagano on the analysis of anthraquinones in leaves from Rhamnaceae species found in the Bergen Swamp. After the summer, he started working with Christina Goudreau Collision on the total synthesis of Trocheliophorolide A. He is currently pursuing a Master’s degree in Chemistry at RIT.
Intended Audience:
Undergraduates, graduates, and experts. Those with interest in the topic.
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