Life Sciences Seminar: Applications of Cryopreserved Human Dissociated Tumor Cells
Life Sciences Seminar
Applications of Cryopreserved Human Dissociated Tumor Cells (DTCs) for Drug and Diagnostic Development
Shawn Fahl ’05
Vice President, Lab Operations, Cell Services & R&D Biospecimens
Discovery Life Sciences
RIT Biotechnology Alum
Abstract:
The acquisition of fresh human tissue is often an impediment to significant research advances. We have developed cryopreserved dissociated human tissues as a viable specimen source for many downstream applications, including immunophenotyping, bulk and single cell sequencing, and single cell functionality. Extensive cellular profiling of these samples by flow cytometry revealed indication-specific trends in the composition of the tumor microenvironment, and these specimens provide the opportunity to screen for novel drug targets and biomarkers. We have used the PD1/PDL1 pathway, which is currently therapeutically targeted in numerous solid cancers, to confirm the utility of these samples to evaluate immunomodulatory pathways. These studies have been extended to uncover novel functional immune receptor to target for the next generation of immunotherapies.
Speaker Bio:
Dr. Shawn Fahl, is a skilled immunologist working to advance the cellular analytical pipelines and R&D laboratory processes for Discovery Life Sciences. Dr. Fahl received his Ph.D. in Microbiology from the University of Virginia in 2013 and his postdoctoral training at Fox Chase Cancer Center from 2013-2017 where he focused on the transcriptional regulation of lymphocyte development and function. An expert in flow cytometric analyses evaluating lymphocyte biology, Dr. Fahl joined Discovery Life Sciences in June 2017 to advance the analytical workflows of dissociated tissue cells. As part of these efforts, Dr. Fahl has overseen the dissociation and flow cytometric analyses of over 5000 tissue samples and the implementation of single cell genomics applications for dissociated tissue. Dr. Fahl has published numerous research articles in prominent scientific journals and presented at national and international conferences.
Intended Audience:
Beginners, undergraduates, graduates. Those with interest in the topic.
To request an interpreter, please visit myaccess.rit.edu
Event Snapshot
When and Where
Who
Open to the Public
Interpreter Requested?
No